Guca2a ELISA Kit (Mouse) (OKCD01908)

Catalog No: OKCD01908 (Formerly GWB-KBBME6)

Size:96WELLS

Mouse Guca2a ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	92-99%	78-99%	96-105%	87-103%
  EDTA Plasma (n=5)	94-103%	96-103%	87-95%	90-103%
  Heparin Plasma (n=5)	96-104%	80-90%	94-105%	85-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase Activator 2A (GUCA2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase Activator 2A (GUCA2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase Activator 2A (GUCA2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Guanylate Cyclase Activator 2A (GUCA2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	91-105	99
  EDTA Plasma (n=5)	78-95	87
  Heparin Plasma (n=5)	92-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanylate Cyclase Activator 2A (GUCA2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanylate Cyclase Activator 2A (GUCA2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Guca2a Microplate: 96 Wells (12 x 8 Well Strips)
  • Guca2a Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated Guca2a Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 3.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Guanylate Cyclase Activator 2A (GUCA2A). No significant cross-reactivity or interference between Guanylate Cyclase Activator 2A (GUCA2A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Guca2a
• Gene Full Name: guanylate cyclase activator 2a (guanylin)
• Alias Symbols: guanylate cyclase activator 2 (guanylin 2, intestinal, heatstable);Guanylate cyclase activator 2A;guanylin;Guca;Guca2.
• NCBI Gene Id: 14915
• Protein Name: Guanylin
• Description of Target: Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins.
• Uniprot ID: P33680
• Protein Accession #: NP_032216.1
• Nucleotide Accession #: NM_008190.1
• Protein Size (# AA): 116