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Catalog No: OKCA00915
Size:96 Wells
Price: $754.00
SKU
OKCA00915
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9086-95
1:210097-104
1:49485-97
1:89891-103
ELISA Kit PrincipleAviva Systems Biology Gpx4 ELISA Kit (Mouse) (OKCA00915) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Gpx4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpx4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Gpx4 captured in well.
ELISA Kit Range31.25 - 2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9589-98
EDTA Plasma (n=4)9287-98
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Gpx4 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Gpx4 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
Gpx4 Microplate96 Wells (12 x 8 Well strips)
Gpx4 Lyophilized Standard2
100X Biotinylated Gpx4 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity< 7.81 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolGPX4
Gene Full NameGlutathione peroxidase 4
Alias Symbolssn, PHG, GPx-4, PHGPx, mtPHG, snGPx, GSHPx-4, mtPHGPx
NCBI Gene Id625249
Protein NamePhospholipid hydroperoxide glutathione peroxidase, nuclear
Description of TargetThe protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. Pseudogenes of this locus have been identified on chromosomes 10 and 17.
Uniprot IDQ91XR9
Protein Accession #NP_001032830.2
Nucleotide Accession #NM_001037741.3
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