Catalog No: OKCD01837 (Formerly GWB-KBBJU6)
Size:96WELLS
Price: $675.00
SKU
OKCD01837
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Datasheets/ManualsPrintable datasheet for GRIA1 ELISA Kit (Human) (OKCD01837)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GRIA1 Microplate96 Wells (12 x 8 Well Strips)
GRIA1 Lyophilized Standard2 x 10 ng
100X Biotinylated GRIA1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGRIA1
Gene Full Nameglutamate ionotropic receptor AMPA type subunit 1
Alias SymbolsAMPA 1;AMPA-selective glutamate receptor 1;GluA1;GLUH1;gluR-1;GLUR1;gluR-A;GLURA;gluR-K1;glutamate receptor 1;Glutamate receptor ionotropic, AMPA 1;glutamate receptor, ionotropic, AMPA 1;HBGR1.
NCBI Gene Id2890
Protein NameGlutamate receptor 1
Description of TargetIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Uniprot IDP42261
Protein Accession #NP_000818.2
Nucleotide Accession #NM_000827.3
Protein Size (# AA)906
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