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Catalog No: OKCD01723 (Formerly GWB-KBBBX1)
Size:96WELLS
Price: $675.00
SKU
OKCD01723
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-92%84-101%78-96%90-98%
EDTA Plasma (n=5)96-103%87-98%94-102%81-92%
Heparin Plasma (n=5)84-91%80-90%91-101%93-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone 2 (GH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone 2 (GH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone 2 (GH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Growth Hormone 2 (GH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)89-10395
EDTA Plasma (n=5)83-10291
Heparin Plasma (n=5)79-9584
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone 2 (GH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone 2 (GH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-GH2 Microplate96 Wells (12 x 8 Well Strips)
GH2 Lyophilized Standard2 x 5 ng
100X Biotinylated GH2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 32 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Hormone 2 (GH2). No significant cross-reactivity or interference between Growth Hormone 2 (GH2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolGH2
Gene Full Namegrowth hormone 2
Alias SymbolsGHB2;GHL;GHV;GH-V;Growth hormone 2;growth hormone B2;growth hormone variant;hGH-V;placental-specific growth hormone;placenta-specific growth hormone.
NCBI Gene Id2689
Protein NameGrowth hormone variant
Description of TargetPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Uniprot IDP01242
Protein Accession #NP_002050.1
Nucleotide Accession #NM_002059.4
Protein Size (# AA)217
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