Fgf6 ELISA Kit (Rat) (OKCD01442)

Catalog No: OKCD01442 (Formerly GWB-KBBCT5)

Size:96WELLS

Rat Fgf6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	81-92%	97-105%	80-98%	85-95%
  EDTA Plasma (n=5)	86-95%	82-104%	95-102%	78-97%
  Heparin Plasma (n=5)	87-104%	86-95%	79-94%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 6 (FGF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 6 (FGF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 6 (FGF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 6 (FGF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-96	90
  EDTA Plasma (n=5)	82-95	88
  Heparin Plasma (n=5)	93-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fgf6 Microplate: 96 Wells (12 x 8 Well Strips)
  • Fgf6 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Fgf6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 6.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 6 (FGF6). No significant cross-reactivity or interference between Fibroblast Growth Factor 6 (FGF6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fgf6
• Gene Full Name: fibroblast growth factor 6
• Alias Symbols: Fgf7b;fibroblast growth factor 6;fibroblast growth factor 7b.
• NCBI Gene Id: 170700
• Protein Name: Fibroblast growth factor
• Description of Target: Fibroblast growth factor
• Uniprot ID: Q8R5L5
• Protein Accession #: NP_571983.1
• Nucleotide Accession #: NM_131908.1
• Protein Size (# AA): 208