Fgf18 ELISA Kit (Rat) (OKCD01714)

Catalog No: OKCD01714 (Formerly GWB-KBBJL8)

Size:96WELLS

Rat Fgf18 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	79-101%	81-91%	86-98%	81-96%
  EDTA Plasma (n=5)	94-101%	80-98%	84-98%	85-99%
  Heparin Plasma (n=5)	97-104%	79-93%	88-102%	79-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 18 (FGF18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 18 (FGF18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 18 (FGF18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 18 (FGF18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	84-91	87
  EDTA Plasma (n=5)	83-93	88
  Heparin Plasma (n=5)	80-99	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 18 (FGF18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fgf18 Microplate: 96 Wells (12 x 8 Well Strips)
  • Fgf18 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Fgf18 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 18 (FGF18). No significant cross-reactivity or interference between Fibroblast Growth Factor 18 (FGF18) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fgf18
• Gene Full Name: fibroblast growth factor 18
• Alias Symbols: FGF-18;fibroblast growth factor 18.
• NCBI Gene Id: 29369
• Protein Name: Fibroblast growth factor 18
• Description of Target: Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation (By similarity).
• Uniprot ID: O88182
• Protein Accession #: NP_062072.1
• Nucleotide Accession #: NM_019199.1
• Protein Size (# AA): 207