Fga ELISA Kit (Mouse) (OKCD01569)

Catalog No: OKCD01569 (Formerly GWB-KBBFO4)

Size:96WELLS

Mouse Fga ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Sodium Citrate Plasma (n=5)	97-105%	79-98%	96-105%	87-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Alpha Chain (FGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Alpha Chain (FGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Alpha Chain (FGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibrinogen Alpha Chain (FGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.25-2000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Sodium Citrate Plasma (n=5)	95-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Alpha Chain (FGA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Alpha Chain (FGA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fga Microplate: 96 Wells (12 x 8 Well Strips)
  • Fga Lyophilized Standard: 2 x 2,000 ng
  • 100X Biotinylated Fga Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 10.5 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibrinogen Alpha (FGa). No significant cross-reactivity or interference between Fibrinogen Alpha (FGa) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fga
• Gene Full Name: fibrinogen alpha chain
• Alias Symbols: Fi;Fib;fibrinogen alpha chain;fibrinogen, A alpha polypeptide.
• NCBI Gene Id: 14161
• Protein Name: Fibrinogen alpha chain
• Description of Target: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots (PubMed:7649481). In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization (PubMed:11389004). Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood (PubMed:7649481). However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo (PubMed:10930441). Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway (PubMed:19332769). Maternal fibrinogen is essential for successful pregnancy (PubMed:7649481). Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage (PubMed:12629066). May also facilitate the immune response via both innate and T-cell mediated pathways (PubMed:23487423).
• Uniprot ID: E9PV24
• Protein Accession #: NP_001104518.1
• Nucleotide Accession #: NM_001111048.2
• Protein Size (# AA): 789