EPHA2 ELISA Kit (Human) (OKCA00672)

Catalog No: OKCA00672

Size:96 Wells

Human EPHA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	90	85-97
  1:2	100	95-105
  1:4	94	89-99
  1:8	95	90-100

• ELISA Kit Principle: Aviva Systems Biology EPHA2 ELISA Kit (Human) (OKCA00672) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPHA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EPHA2 captured in the well.
• ELISA Kit Range: 0.156 ng/mL-10 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96	89-101
  EDTA Plasma (n=4)	92	85-99

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level EPHA2 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level EPHA2 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • EPHA2 Microplate: 96 Wells (12 x 8 Well strips)
  • EPHA2 Lyophilized Standard: 2
  • 100X Biotinylated EPHA2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates And Cell Lysates
• Sensitivity: 0.039 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EPHA2
• Gene Full Name: EPH receptor A2
• Alias Symbols: ECK, CTPA, ARCC2, CTPP1, CTRCT6
• NCBI Gene Id: 1969
• Protein Name: Ephrin type-A receptor 2
• Description of Target: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
• Uniprot ID: P29317
• Protein Accession #: NP_004422.2
• Nucleotide Accession #: NM_004431.4