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Catalog No: OKCD02015 (Formerly GWB-KBBGX8)
Size:96WELLS
Price: $675.00
SKU
OKCD02015
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Datasheets/ManualsPrintable datasheet for ELAVL1 ELISA Kit (Human) (OKCD02015)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELAV Like Protein 1 (ELAVL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ELAV Like Protein 1 (ELAVL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELAV Like Protein 1 (ELAVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ELAV Like Protein 1 (ELAVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ELAVL1 Microplate96 Wells (12 x 8 Well Strips)
ELAVL1 Lyophilized Standard2 x 20 ng
100X Biotinylated ELAVL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of ELAV Like Protein 1 (ELAVL1). No significant cross-reactivity or interference between ELAV Like Protein 1 (ELAVL1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolELAVL1
Gene Full NameELAV like RNA binding protein 1
Alias SymbolsELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R);ELAV1;ELAV-like protein 1;embryonic lethal, abnormal vision, drosophila, homolog-like 1;Hu antigen R;Hua;hu-antigen R;HUR;MelG.
NCBI Gene Id1994
Protein NameELAV-like protein 1
Description of TargetRNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity).
Uniprot IDQ15717
Protein Accession #NP_001410.2
Nucleotide Accession #NM_001419.2
Protein Size (# AA)326
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