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Catalog No: OKCD01868 (Formerly GWB-KBBDN8)
Size:96WELLS
Price: $675.00
SKU
OKCD01868
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Datasheets/ManualsPrintable datasheet for EFEMP2 ELISA Kit (Human) (OKCD01868)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)81-95%93-102%88-95%94-101%
EDTA Plasma (n=5)85-101%97-105%89-96%95-104%
Heparin Plasma (n=5)99-105%94-102%91-98%82-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 4 (FBLN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 4 (FBLN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 4 (FBLN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibulin 4 (FBLN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)94-10299
EDTA Plasma (n=5)86-9589
Heparin Plasma (n=5)96-10399
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 4 (FBLN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 4 (FBLN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EFEMP2 Microplate96 Wells (12 x 8 Well Strips)
EFEMP2 Lyophilized Standard2 x 200 ng
100X Biotinylated EFEMP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 1.32 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibulin 4 (FBLN4). No significant cross-reactivity or interference between Fibulin 4 (FBLN4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEFEMP2
Gene Full NameEGF containing fibulin extracellular matrix protein 2
Alias SymbolsARCL1B;EGF containing fibulin like extracellular matrix protein 2;EGF-containing fibulin-like extracellular matrix protein 2;FBLN4;FIBL-4;fibulin 4;Fibulin-4;MBP1;mutant p53 binding protein 1;Protein UPH1;UPH1.
NCBI Gene Id30008
Protein NameEGF-containing fibulin-like extracellular matrix protein 2
Description of TargetEGF-containing fibulin-like extracellular matrix protein 2
Uniprot IDO95967
Protein Accession #NP_058634.4
Nucleotide Accession #NM_016938.4
Protein Size (# AA)443
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