EDA2R ELISA Kit (Human) (OKCD01792)

Catalog No: OKCD01792 (Formerly GWB-KBBQG4)

Size:96WELLS

Human EDA2R ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-90%	95-103%	79-91%	84-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectodysplasin A2 Receptor (EDA2R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectodysplasin A2 Receptor (EDA2R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectodysplasin A2 Receptor (EDA2R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ectodysplasin A2 Receptor (EDA2R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ectodysplasin A2 Receptor (EDA2R) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ectodysplasin A2 Receptor (EDA2R) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EDA2R Microplate: 96 Wells (12 x 8 Well Strips)
  • EDA2R Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated EDA2R Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ectodysplasin A2 Receptor (EDA2R). No significant cross-reactivity or interference between Ectodysplasin A2 Receptor (EDA2R) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: EDA2R
• Gene Full Name: ectodysplasin A2 receptor
• Alias Symbols: EDA-A2 receptor;EDAA2R;EDA-A2R;TNFRSF27;tumor necrosis factor receptor superfamily member 27;tumor necrosis factor receptor superfamily member XEDAR;XEDAR;X-linked ectodysplasin-A2 receptor.
• NCBI Gene Id: 60401
• Protein Name: Tumor necrosis factor receptor superfamily member 27
• Description of Target: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6.
• Uniprot ID: Q9HAV5
• Protein Accession #: NP_001186616.1
• Nucleotide Accession #: NM_001199687.2
• Protein Size (# AA): 297