DEFA3 ELISA Kit (Human) (OKCD01808)

Catalog No: OKCD01808 (Formerly GWB-KBBJV4)

Size:96WELLS

Human DEFA3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-103%	80-92%	86-95%	82-97%
  EDTA Plasma (n=5)	82-93%	98-105%	97-105%	81-104%
  Heparin Plasma (n=5)	78-97%	84-104%	90-98%	87-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 3, Neutrophil Specific (DEFa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 3, Neutrophil Specific (DEFa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 3, Neutrophil Specific (DEFa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Alpha 3, Neutrophil Specific (DEFa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.47-30ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	95-105	101
  EDTA Plasma (n=5)	78-90	86
  Heparin Plasma (n=5)	88-97	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Alpha 3, Neutrophil Specific (DEFa3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Alpha 3, Neutrophil Specific (DEFa3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DEFA3 Microplate: 96 Wells (12 x 8 Well Strips)
  • DEFA3 Lyophilized Standard: 2 x 30 ng
  • 100X Biotinylated DEFA3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.21 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Defensin Alpha 3, Neutrophil Specific (DEFa3). No significant cross-reactivity or interference between Defensin Alpha 3, Neutrophil Specific (DEFa3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DEFA3
• Gene Full Name: defensin alpha 3
• Alias Symbols: DEF3;defensin 3, neutrophil-specific;Defensin, alpha 3;defensin, alpha 3, neutrophil-specific;HNP3;HNP-3;HP3;HP-3;neutrophil defensin 3;neutrophil peptide 3.
• NCBI Gene Id: 1668
• Protein Name: Neutrophil defensin 3
• Description of Target: Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
• Uniprot ID: P59666
• Protein Accession #: NP_005208.1
• Nucleotide Accession #: NM_005217.3
• Protein Size (# AA): 94