Catalog No: OKCD01675 (Formerly GWB-KBBAV6)
Size:96WELLS
Price: $675.00
SKU
OKCD01675
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Datasheets/ManualsPrintable datasheet for DCX ELISA Kit (Human) (OKCD01675)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Doublecortin (DCX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Doublecortin (DCX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Doublecortin (DCX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Doublecortin (DCX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Doublecortin (DCX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Doublecortin (DCX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DCX Microplate96 Wells (12 x 8 Well Strips)
DCX Lyophilized Standard2 x 10 ng
100X Biotinylated DCX Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Doublecortin (DCX). No significant cross-reactivity or interference between Doublecortin (DCX) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDCX
Gene Full Namedoublecortin
Alias SymbolsDBCN;DC;doublecortex;doublin;lissencephalin-X;lis-X;LISX;neuronal migration protein doublecortin;SCLH;XLIS.
NCBI Gene Id1641
Protein NameNeuronal migration protein doublecortin
Description of TargetMicrotubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Uniprot IDO43602
Protein Accession #NP_000546.2
Nucleotide Accession #NM_000555.3
Protein Size (# AA)365
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