DAO ELISA Kit (Rat) (OKCA00969)

Catalog No: OKCA00969

Size:96 Wells

Rat DAO ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:100	85	80-91
  1:200	93	89-99
  1:400	87	82-95
  1:800	101	98-104

• ELISA Kit Principle: Aviva Systems Biology DAO ELISA Kit (Rat) (OKCA00969) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for DAO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DAO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DAO captured in well.
• ELISA Kit Range: 0.78 mIU/mL-50 mIU/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85	81-92
  EDTA Plasma (n=4)	90	85-96

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level DAO were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level DAO were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • DAO Microplate: 96 Wells (12 x 8 Well strips)
  • DAO Lyophilized Standard: 2
  • 100X Biotinylated DAO Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates
• Sensitivity: 0.195 mIU/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DAO
• Gene Full Name: D-amino-acid oxidase
• NCBI Gene Id: 100008977
• Protein Name: D-amino-acid oxidase
• Description of Target: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
• Uniprot ID: P22942
• Protein Accession #: NP_001075658.1
• Nucleotide Accession #: NM_001082189.1