Catalog No: OKCD01676 (Formerly GWB-KBBDK2)
Size:96WELLS
Price: $675.00
SKU
OKCD01676
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Datasheets/ManualsPrintable datasheet for DAG1 ELISA Kit (Human) (OKCD01676)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-105%98-105%95-105%82-101%
EDTA Plasma (n=5)92-105%78-101%91-105%87-94%
Heparin Plasma (n=5)83-99%97-104%78-95%97-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrophin Associated Glycoprotein 1 (DAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dystrophin Associated Glycoprotein 1 (DAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9892
EDTA Plasma (n=5)85-9990
Heparin Plasma (n=5)80-9386
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DAG1 Microplate96 Wells (12 x 8 Well Strips)
DAG1 Lyophilized Standard2 x 20 ng
100X Biotinylated DAG1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.126 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dystrophin Associated Glycoprotein 1 (DAG1). No significant cross-reactivity or interference between Dystrophin Associated Glycoprotein 1 (DAG1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDAG1
Gene Full Namedystroglycan 1
Alias Symbols156DAG;A3a;AGRNR;DAG;dystroglycan;dystroglycan 1 (dystrophin-associated glycoprotein 1);Dystrophin-associated glycoprotein 1;LGMDR16;MDDGA9;MDDGC7;MDDGC9.
NCBI Gene Id1605
Protein NameDystroglycan
Description of TargetThe dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Uniprot IDQ14118
Protein Accession #NP_001159400.2
Nucleotide Accession #NM_001165928.3
Protein Size (# AA)895
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