COL18A1 ELISA Kit (Rat) (OKCA00970)

Catalog No: OKCA00970

Size:96 Wells

Rat COL18A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	89	85-94
  1:2	101	97-104
  1:4	90	87-94
  1:8	100	95-103

• ELISA Kit Principle: Aviva Systems Biology Col18a1 ELISA Kit (Rat) (OKCA00970) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Col18a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Col18a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Col18a1 captured in well.
• ELISA Kit Range: 6.25 ng/mL-400 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94	91-98
  EDTA Plasma (n=4)	95	92-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Col18a1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Col18a1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Col18a1 Microplate: 96 Wells (12 x 8 Well strips)
  • Col18a1 Lyophilized Standard: 2
  • 100X Biotinylated Col18a1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.56 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: COL18A1
• Gene Full Name: Collagen type XVIII alpha 1 chain
• NCBI Gene Id: 85251
• Protein Name: Collagen type XVIII alpha 1 chain
• Uniprot ID: Q9WUW5
• Protein Accession #: NP_445941.2
• Nucleotide Accession #: NM_053489.2