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Catalog No: OKCD01863 (Formerly GWB-KBBMH6)
Size:96WELLS
Price: $725.00
SKU
OKCD01863
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Datasheets/ManualsPrintable datasheet for Cldn5 ELISA Kit (Rat) (OKCD01863)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5 (CLDN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 5 (CLDN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 5 (CLDN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Claudin 5 (CLDN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 5 (CLDN5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 5 (CLDN5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cldn5 Microplate96 Wells (12 x 8 Well Strips)
Cldn5 Lyophilized Standard2 x 20 ng
100X Biotinylated Cldn5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Claudin 5 (CLDN5). No significant cross-reactivity or interference between Claudin 5 (CLDN5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCldn5
Gene Full Nameclaudin 5
Alias Symbolsclaudin-5.
NCBI Gene Id65131
Protein NameClaudin-5
Description of TargetPlays a major role in tight junction-specific obliteration of the intercellular space.
Uniprot IDQ9JKD6
Protein Accession #NP_113889.1
Nucleotide Accession #NM_031701.2
Protein Size (# AA)218
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