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Catalog No: OKCD01603 (Formerly GWB-KBBQE1)
Size:96WELLS
Price: $650.00
SKU
OKCD01603
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Datasheets/ManualsPrintable datasheet for CYSLTR2 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteinyl Leukotriene Receptor 2 (CYSLTR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteinyl Leukotriene Receptor 2 (CYSLTR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteinyl Leukotriene Receptor 2 (CYSLTR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 2 (CYSLTR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteinyl Leukotriene Receptor 2 (CYSLTR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYSLTR2 Microplate96 Wells (12 x 8 Well Strips)
CYSLTR2 Lyophilized Standard2 x 20 ng
100X Biotinylated CYSLTR2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.122 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cysteinyl Leukotriene Receptor 2 (CYSLTR2). No significant cross-reactivity or interference between Cysteinyl Leukotriene Receptor 2 (CYSLTR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCYSLTR2
Gene Full NameCysteinyl leukotriene receptor 2
Alias SymbolsHG57, CYSLT2, GPCR21, HPN321, CYSLT2R, KPG_011, hGPCR21, PSEC0146
NCBI Gene Id57105
Protein NameCysteinyl leukotriene receptor 2
Description of TargetReceptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4 >> LTE4.
Uniprot IDQ9NS75
Protein Accession #NP_001295394.1
Nucleotide Accession #NM_001308465.1
Protein Size (# AA)346
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