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Catalog No: OKCD01437 (Formerly GWB-KBBUX4)
Size:96WELLS
Price: $650.00
SKU
OKCD01437
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Datasheets/ManualsPrintable datasheet for CNTF ELISA Kit (Rabbit) (OKCD01437)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-96%80-101%78-102%87-101%
EDTA Plasma (n=5)93-104%80-95%89-98%90-97%
Heparin Plasma (n=5)84-104%98-105%88-97%96-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ciliary Neurotrophic Factor (CNTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ciliary Neurotrophic Factor (CNTF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)90-9995
EDTA Plasma (n=5)78-9488
Heparin Plasma (n=5)95-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CNTF Microplate96 Wells (12 x 8 Well Strips)
CNTF Lyophilized Standard2 x 2 ng
100X Biotinylated CNTF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 11.8 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ciliary Neurotrophic Factor (CNTF). No significant cross-reactivity or interference between Ciliary Neurotrophic Factor (CNTF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCNTF
Gene Full Nameciliary neurotrophic factor
Alias Symbolsciliary neurotrophic factor;ciliary neurotropic factor.
NCBI Gene Id100009117
Protein NameCiliary neurotrophic factor
Description of TargetCNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Uniprot IDP14188
Protein Accession #NP_001075752.1
Nucleotide Accession #NM_001082283.1
Protein Size (# AA)199
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