CLEC2D ELISA Kit (Human) (OKCA00652)

Catalog No: OKCA00652

Size:96 Wells

Human CLEC2D ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	89	85-93
  1:2	95	92-99
  1:4	87	83-91
  1:8	90	84-97

• ELISA Kit Principle: Aviva Systems Biology CLEC2D ELISA Kit (Human) (OKCA00652) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC2D has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC2D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CLEC2D captured in the well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92	87-97
  EDTA Plasma (n=4)	96	90-101

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CLEC2D were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CLEC2D were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • CLEC2D Microplate: 96 Wells (12 x 8 Well strips)
  • CLEC2D Lyophilized Standard: 2
  • 100X Biotinylated CLEC2D Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.81 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CLEC2D
• Gene Full Name: C-type lectin domain family 2 member D
• Alias Symbols: CLAX, LLT1, OCIL
• NCBI Gene Id: 29121
• Protein Name: C-type lectin domain family 2 member D
• Description of Target: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.
• Uniprot ID: Q9UHP7
• Protein Accession #: NP_001004419.1
• Nucleotide Accession #: NM_001004419.4