Catalog No: OKCD01861 (Formerly GWB-KBBDM6)
Size:96WELLS
Price: $675.00
SKU
OKCD01861
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Datasheets/ManualsPrintable datasheet for CLDN5 ELISA Kit (Human) (OKCD01861)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)99-105%86-104%93-101%81-89%
EDTA Plasma (n=5)87-101%94-101%90-99%78-89%
Heparin Plasma (n=5)78-104%81-98%78-96%87-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5 (CLDN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 5 (CLDN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 5 (CLDN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Claudin 5 (CLDN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-104101
EDTA Plasma (n=5)81-9188
Heparin Plasma (n=5)96-103101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 5 (CLDN5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 5 (CLDN5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CLDN5 Microplate96 Wells (12 x 8 Well Strips)
CLDN5 Lyophilized Standard2 x 2 ng
100X Biotinylated CLDN5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 13.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Claudin 5 (CLDN5). No significant cross-reactivity or interference between Claudin 5 (CLDN5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCLDN5
Gene Full Nameclaudin 5
Alias SymbolsAWAL;BEC1;claudin-5;CPETRL1;TMDVCF;TMVCF;transmembrane protein deleted in VCFS;transmembrane protein deleted in velocardiofacial syndrome.
NCBI Gene Id7122
Protein NameClaudin-5
Description of TargetPlays a major role in tight junction-specific obliteration of the intercellular space.
Uniprot IDO00501
Protein Accession #NP_001124333.1
Nucleotide Accession #NM_001130861.1
Protein Size (# AA)218
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