CHST7 ELISA Kit (Human) (OKCD01999)

Catalog No: OKCD01999 (Formerly GWB-KBBYY9)

Size:96WELLS

Human CHST7 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	82-104%	79-91%	91-98%	79-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Sulfotransferase 7 (CHST7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbohydrate Sulfotransferase 7 (CHST7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Sulfotransferase 7 (CHST7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbohydrate Sulfotransferase 7 (CHST7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	89-103	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbohydrate Sulfotransferase 7 (CHST7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbohydrate Sulfotransferase 7 (CHST7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CHST7 Microplate: 96 Wells (12 x 8 Well Strips)
  • CHST7 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated CHST7 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 31 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carbohydrate Sulfotransferase 7 (CHST7). No significant cross-reactivity or interference between Carbohydrate Sulfotransferase 7 (CHST7) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CHST7
• Gene Full Name: carbohydrate sulfotransferase 7
• Alias Symbols: C6ST-2;carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7;carbohydrate sulfotransferase 7;Chondroitin 6-sulfotransferase 2;chondroitin 6-sulfotransferase-2;galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5;glcNAc6ST-4;gn6st-4;GST-5;N-acetylglucosamine 6-O-sulfotransferase 4.
• NCBI Gene Id: 56548
• Protein Name: Carbohydrate sulfotransferase 7
• Description of Target: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.
• Uniprot ID: Q9NS84
• Protein Accession #: NP_063939.2
• Nucleotide Accession #: NM_019886.3
• Protein Size (# AA): 486