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Catalog No: OKCD01532 (Formerly GWB-KBBTN5)
Size:96WELLS
Price: $650.00
SKU
OKCD01532
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Datasheets/ManualsPrintable datasheet for CFH ELISA Kit (Human) (OKCD01532)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-102%79-89%95-105%96-105%
EDTA Plasma (n=5)80-102%95-105%84-101%97-105%
Heparin Plasma (n=5)87-101%82-94%84-99%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H (CFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H (CFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H (CFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-10598
EDTA Plasma (n=5)87-9491
Heparin Plasma (n=5)83-9187
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H (CFH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H (CFH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CFH Microplate96 Wells (12 x 8 Well Strips)
CFH Lyophilized Standard2 x 300 ng
100X Biotinylated CFH Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 1.9 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Complement Factor H (CFH). No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCFH
Gene Full Namecomplement factor H
Alias Symbolsadrenomedullin binding protein;age-related maculopathy susceptibility 1;AHUS1;AMBP1;ARMD4;ARMS1;beta-1H;beta-1-H-globulin;CFHL3;complement factor H;factor H;factor H-like 1;FH;FHL1;H factor 1;H factor 1 (complement);H factor 2 (complement);HF;HF1;HF2;HUS.
NCBI Gene Id3075
Protein NameComplement factor H
Description of TargetFactor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
Uniprot IDP08603
Protein Accession #NP_000177.2
Nucleotide Accession #NM_000186.3
Protein Size (# AA)1231
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