Catalog No: OKCD01666 (Formerly GWB-KBBNH3)
Size:96WELLS
Price: $675.00
SKU
OKCD01666
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Datasheets/ManualsPrintable datasheet for CDX2 ELISA Kit (Human) (OKCD01666)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caudal Type Homeobox 2 (CDX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caudal Type Homeobox 2 (CDX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caudal Type Homeobox 2 (CDX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Caudal Type Homeobox 2 (CDX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caudal Type Homeobox 2 (CDX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caudal Type Homeobox 2 (CDX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CDX2 Microplate96 Wells (12 x 8 Well Strips)
CDX2 Lyophilized Standard2 x 10 ng
100X Biotinylated CDX2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Caudal Type Homeobox Transcription Factor 2 (CDX2). No significant cross-reactivity or interference between Caudal Type Homeobox Transcription Factor 2 (CDX2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCDX2
Gene Full Namecaudal type homeobox 2
Alias Symbolscaudal type homeobox transcription factor 2;caudal-type homeobox protein 2;CDX2/AS;CDX3;CDX-3;homeobox protein CDX-2;homeobox protein miniCDX2.
NCBI Gene Id1045
Protein NameHomeobox protein CDX-2
Description of TargetInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).
Uniprot IDQ99626
Protein Accession #NP_001256.3
Nucleotide Accession #NM_001265.4
Protein Size (# AA)313
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