CCDC3 ELISA Kit (Human) (OKCD02109)

Catalog No: OKCD02109 (Formerly GWB-KBBHH9)

Size:96WELLS

Human CCDC3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	81-104%	90-104%	90-98%	91-105%
  EDTA Plasma (n=5)	78-96%	80-93%	82-99%	90-97%
  Heparin Plasma (n=5)	79-94%	87-102%	78-96%	82-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coiled Coil Domain Containing Protein 3 (CCDC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coiled Coil Domain Containing Protein 3 (CCDC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coiled Coil Domain Containing Protein 3 (CCDC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coiled Coil Domain Containing Protein 3 (CCDC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	98-105	101
  EDTA Plasma (n=5)	83-97	92
  Heparin Plasma (n=5)	89-101	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coiled Coil Domain Containing Protein 3 (CCDC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coiled Coil Domain Containing Protein 3 (CCDC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CCDC3 Microplate: 96 Wells (12 x 8 Well Strips)
  • CCDC3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CCDC3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coiled Coil Domain Containing Protein 3 (CCDC3). No significant cross-reactivity or interference between Coiled Coil Domain Containing Protein 3 (CCDC3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CCDC3
• Gene Full Name: coiled-coil domain containing 3
• Alias Symbols: coiled-coil domain-containing protein 3;fat/vessel-derived secretory protein;favine.
• NCBI Gene Id: 83643
• Protein Name: Coiled-coil domain-containing protein 3
• Description of Target: Negatively regulates TNF-alpha-induced pro-inflammatory response in endothelial cells (ECs) via inhibition of TNF-alpha-induced NF-kappaB activation in ECs (PubMed:25193116). Positively regulates lipid accumulation in adipose cells (By similarity).
• Uniprot ID: Q9BQI4
• Protein Accession #: NP_001269587.1
• Nucleotide Accession #: NM_001282658.1
• Protein Size (# AA): 270