Catalog No: OKCA00853
Size:96 Wells
Price: $754.00
SKU
OKCA00853
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat8884-92
1:210095-105
1:48783-91
1:8104100-108
ELISA Kit PrincipleAviva Systems Biology C9orf72 ELISA Kit (Human) (OKCA00853) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for C9orf72 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C9orf72 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C9orf72 captured in well.
ELISA Kit Range31.25 - 2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)8683-89
EDTA Plasma (n=4)9189-95
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level C9orf72 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level C9orf72 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
C9orf72 Microplate96 Wells (12 x 8 Well strips)
C9orf72 Lyophilized Standard2
100X Biotinylated C9orf72 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity< 7.81 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolC9ORF72
Gene Full NameChromosome 9 open reading frame 72
Alias SymbolsALSFTD, DENND9, FTDALS, DENNL72, FTDALS1
NCBI Gene Id203228
Protein NameProtein C9orf72
Description of TargetMay play a role in endosomal trafficking and autophagy.
Uniprot IDQ96LT7
Protein Accession #NP_001242983.1
Nucleotide Accession #NM_001256054.2
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