Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKCD01578 (Formerly GWB-KBBVX1)
Size:96WELLS
Price: $650.00
SKU
OKCD01578
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for C5ar1 ELISA Kit (Mouse) (OKCD01578)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a Receptor 1 (C5aR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 5a Receptor 1 (C5aR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5a Receptor 1 (C5aR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 5a Receptor 1 (C5aR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 5a Receptor 1 (C5aR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5a Receptor 1 (C5aR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-C5ar1 Microplate96 Wells (12 x 8 Well Strips)
C5ar1 Lyophilized Standard2 x 20 ng
100X Biotinylated C5ar1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Complement Component 5a Receptor 1 (C5aR1). No significant cross-reactivity or interference between Complement Component 5a Receptor 1 (C5aR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolC5ar1
Gene Full Namecomplement component 5a receptor 1
Alias SymbolsC5;C5a anaphylatoxin chemotactic receptor;C5a anaphylatoxin chemotactic receptor 1;C5a anaphylatoxin receptor;C5a ligand;C5aR;C5a-R;C5r;C5r1;Cd88;complement component 5, receptor 1;D7Msu;D7Msu1.
NCBI Gene Id12273
Protein NameC5a anaphylatoxin chemotactic receptor 1
Description of TargetReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
Uniprot IDP30993
Protein Accession #NP_001167021.1
Nucleotide Accession #NM_001173550.1
Protein Size (# AA)351
Write Your Own Review
You're reviewing:C5ar1 ELISA Kit (Mouse) (OKCD01578)
Your Rating
We found other products you might like!