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Catalog No: OKCA00642
Size:96 Wells
Price: $754.00
SKU
OKCA00642
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9792-101
1:210599-108
1:49084-94
1:88782-91
ELISA Kit PrincipleAviva Systems Biology C1QTNF6 ELISA Kit (Human) (OKCA00642) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1QTNF6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1QTNF6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C1QTNF6 captured in the well.
ELISA Kit Range0.312 ng/mL-20 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9285-96
EDTA Plasma (n=4)9082-93
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level C1QTNF6 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level C1QTNF6 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
C1QTNF6 Microplate96 Wells (12 x 8 Well strips)
C1QTNF6 Lyophilized Standard2
100X Biotinylated C1QTNF6 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolC1QTNF6
Gene Full NameC1q and tumor necrosis factor related protein 6
Alias SymbolsCTFP6, CTRP6, ZACRP6
NCBI Gene Id114904
Protein NameComplement C1q tumor necrosis factor-related protein 6
Uniprot IDQ9BXI9
Protein Accession #NP_114116.3
Nucleotide Accession #NM_031910.3
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