Catalog No: OKCD02111 (Formerly GWB-KBBJY6)
Size:96WELLS
Price: $750.00
SKU
OKCD02111
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Datasheets/ManualsPrintable datasheet for C16orf89 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-96%98-105%79-93%78-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromosome 16 Open Reading Frame 89 (C16orf89). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromosome 16 Open Reading Frame 89 (C16orf89). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromosome 16 Open Reading Frame 89 (C16orf89), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromosome 16 Open Reading Frame 89 (C16orf89) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum79-9588
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromosome 16 Open Reading Frame 89 (C16orf89) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromosome 16 Open Reading Frame 89 (C16orf89) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-C16orf89 Microplate96 Wells (12 x 8 Well Strips)
C16orf89 Lyophilized Standard2 x 10 ng
100X Biotinylated C16orf89 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.060 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Chromosome 16 Open Reading Frame 89 (C16orf89). No significant cross-reactivity or interference between Chromosome 16 Open Reading Frame 89 (C16orf89) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolC16ORF89
Gene Full NameChromosome 16 open reading frame 89
Alias SymbolsMGC45438, UNQ904/PRO1925, UPF0764 protein C16orf89
NCBI Gene Id146556
Protein NameUPF0764 protein C16orf89
Description of TargetThis gene is expressed predominantly in the thyroid. Based on expression patterns similar to thyroid transcription factors and proteins, this gene may function in the development and function of the thyroid. Multiple transcript variants encoding different isoforms have been found for this gene.
Uniprot IDQ6UX73
Protein Accession #NP_001091984.2
Nucleotide Accession #NM_001098514.2
Protein Size (# AA)402
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