Bcl2l1 ELISA Kit (Mouse) (OKCD01826)

Catalog No: OKCD01826 (Formerly GWB-KBBZG7)

Size:96WELLS

Mouse Bcl2l1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.81-500pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Bcl2l1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Bcl2l1 Lyophilized Standard: 2 x 500 pg
  • 100X Biotinylated Bcl2l1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 3.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Bcl2l1
• Gene Full Name: BCL2-like 1
• Alias Symbols: anti-apoptosis regulatory protein;apoptosis regulator Bcl-X;B-cell leukemia/lymphoma x;Bcl;bcl-;Bcl(X;Bcl(X)L;Bcl2l;bcl2-L-1;bcl-2-like protein 1;bcl-x;BclX;Bcl-XL.
• NCBI Gene Id: 12048
• Protein Name: Bcl-2-like protein 1
• Description of Target: Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.
• Uniprot ID: Q64373
• Protein Accession #: NP_001276645.1
• Nucleotide Accession #: NM_001289716.1
• Protein Size (# AA): 233