Catalog No: OKCD02005 (Formerly GWB-KBBBE6)
Size:96WELLS
Price: $675.00
SKU
OKCD02005
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Datasheets/ManualsPrintable datasheet for BLVRA ELISA Kit (Human) (OKCD02005)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-98%80-97%91-105%83-94%
EDTA Plasma (n=5)98-105%97-105%93-105%85-99%
Heparin Plasma (n=5)96-104%98-105%85-98%80-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase A (BLVRA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biliverdin Reductase A (BLVRA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase A (BLVRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Biliverdin Reductase A (BLVRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-8984
EDTA Plasma (n=5)87-9792
Heparin Plasma (n=5)78-9689
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Biliverdin Reductase A (BLVRA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Biliverdin Reductase A (BLVRA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BLVRA Microplate96 Wells (12 x 8 Well Strips)
BLVRA Lyophilized Standard2 x 40 ng
100X Biotinylated BLVRA Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.273 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Biliverdin Reductase A (BLVRA). No significant cross-reactivity or interference between Biliverdin Reductase A (BLVRA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBLVRA
Gene Full Namebiliverdin reductase A
Alias Symbolsbiliverdin reductase A;biliverdin-IX alpha-reductase;BLVR;BVR;BVR A;BVRA;testis tissue sperm-binding protein Li 61n.
NCBI Gene Id644
Protein NameBiliverdin reductase A
Description of TargetReduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
Uniprot IDP53004
Protein Accession #NP_000703.2
Nucleotide Accession #NM_000712.3
Protein Size (# AA)296
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