Avpr2 ELISA Kit (Mouse) (OKCD01660)

Catalog No: OKCD01660 (Formerly GWB-KBBLE6)

Size:96WELLS

Mouse Avpr2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginine Vasopressin Receptor 2 (AVPR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginine Vasopressin Receptor 2 (AVPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arginine Vasopressin Receptor 2 (AVPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine Vasopressin Receptor 2 (AVPR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine Vasopressin Receptor 2 (AVPR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Avpr2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Avpr2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Avpr2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arginine Vasopressin Receptor 2 (AVPR2). No significant cross-reactivity or interference between Arginine Vasopressin Receptor 2 (AVPR2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AVPR2
• Gene Full Name: Arginine vasopressin receptor 2
• Alias Symbols: V, DI, ND, DI1, DIR, ND1, V2R, ADHR, VPV2R
• NCBI Gene Id: 12000
• Protein Name: Vasopressin V2 receptor
• Description of Target: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
• Uniprot ID: O88721
• Protein Accession #: NP_062277.1
• Nucleotide Accession #: NM_019404.2
• Protein Size (# AA): 371