Catalog No: OKCD01750 (Formerly GWB-KBBDQ8)
Size:96WELLS
Price: $700.00
SKU
OKCD01750
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Datasheets/ManualsPrintable datasheet for ATXN10 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxin 10 (ATXN10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ataxin 10 (ATXN10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ataxin 10 (ATXN10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ataxin 10 (ATXN10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ataxin 10 (ATXN10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ataxin 10 (ATXN10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Atxn10 Microplate96 Wells (12 x 8 Well Strips)
Atxn10 Lyophilized Standard2 x 10 ng
100X Biotinylated Atxn10 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ataxin 10 (ATXN10). No significant cross-reactivity or interference between Ataxin 10 (ATXN10) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolATXN10
Gene Full NameAtaxin 10
Alias SymbolsE46, Sca, Sca10, C77170, Tex169, TEG-169, AI325283
NCBI Gene Id54138
Protein NameAtaxin-10
Description of TargetNecessary for the survival of cerebellar neurons. Induces neuritogenesis by activating the Ras-MAP kinase pathway. May play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.
Uniprot IDP28658
Protein Accession #NP_058539.2
Nucleotide Accession #NM_016843.3
Protein Size (# AA)475
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