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Catalog No: OKCD02107 (Formerly GWB-KBBRF6)
Size:96WELLS
Price: $750.00
SKU
OKCD02107
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Datasheets/ManualsPrintable datasheet for ASCC3 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)93-101%84-98%87-101%84-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum91-10598
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ASCC3 Microplate96 Wells (12 x 8 Well Strips)
ASCC3 Lyophilized Standard2 x 20 ng
100X Biotinylated ASCC3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.122 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). No significant cross-reactivity or interference between Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolASCC3
Gene Full NameActivating signal cointegrator 1 complex subunit 3
Alias SymbolsRNAH, HELIC1, ASC1p200
NCBI Gene Id10973
Protein NameActivating signal cointegrator 1 complex subunit 3
Description of Target3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Enhances NF-kappa-B, SRF and AP1 transactivation.
Uniprot IDQ8N3C0
Protein Accession #NP_001271200.1
Nucleotide Accession #NM_001284271.1
Protein Size (# AA)2203
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