AS3MT ELISA Kit (Human) (OKCD02008)

Catalog No: OKCD02008 (Formerly GWB-KBBEG7)

Size:96WELLS

Human AS3MT ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arsenic, 3-Oxidation State Methyltransferase (AS3MT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arsenic, 3-Oxidation State Methyltransferase (AS3MT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arsenic, 3-Oxidation State Methyltransferase (AS3MT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arsenic, 3-Oxidation State Methyltransferase (AS3MT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arsenic, 3-Oxidation State Methyltransferase (AS3MT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arsenic, 3-Oxidation State Methyltransferase (AS3MT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CILP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CILP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CILP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 11 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arsenic, 3-Oxidation State Methyltransferase (AS3MT). No significant cross-reactivity or interference between Arsenic, 3-Oxidation State Methyltransferase (AS3MT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AS3MT
• Gene Full Name: Arsenite methyltransferase
• Alias Symbols: CYT19
• NCBI Gene Id: 57412
• Protein Name: Arsenite methyltransferase
• Description of Target: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH.
• Uniprot ID: Q9HBK9
• Protein Accession #: NP_065733.2
• Nucleotide Accession #: NM_020682.3
• Protein Size (# AA): 375