ANPEP ELISA Kit (Rat) (OKCA00944)

Catalog No: OKCA00944

Size:96 Wells

Rat ANPEP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	90	83-97
  1:2	94	90-98
  1:4	106	103-109
  1:8	85	82-88

• ELISA Kit Principle: Aviva Systems Biology ANPEP ELISA Kit (Rat) (OKCA00944) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for ANPEP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANPEP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ANPEP captured in well.
• ELISA Kit Range: 0.312 ng/mL-20 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93	90-96
  EDTA Plasma (n=4)	105	103-107

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level ANPEP were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level ANPEP were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • ANPEP Microplate: 96 Wells (12 x 8 Well strips)
  • ANPEP Lyophilized Standard: 2
  • 100X Biotinylated ANPEP Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANPEP
• Gene Full Name: Aminopeptidase N
• Alias Symbols: APN
• NCBI Gene Id: 100009167
• Protein Name: Aminopeptidase N
• Description of Target: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis.
• Uniprot ID: P15541
• Protein Accession #: NP_001075795.1
• Nucleotide Accession #: NM_001082326.1