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Catalog No: OKEH02553
Size:96T
Price: $625.00
SKU
OKEH02553
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02553
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology 8OHDG ELISA Kit (OKEH02553) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-8OHDG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 8OHDG and incubated. The 8OHDG found in the sample or standards competes with the biotinylated 8OHDG for limited binding sites on the immobilized anti-8OHDG antibody. Excess unbound biotinylated 8OHDG and sample or standard 8OHDG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 8OHDG captured in the well and inversely proportional to the amount of 8OHDG which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 85%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-8OHDG Microplate96 Wells (12 x 8 Well strips)
8OHDG Lyophilized Standard2 x 50 ng
Sample Diluent1 x 20 mL
100X 8OHDG-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: 8OHDG
Target Name: 8-Oxo-2'-deoxyguanosine
::Pubchem: 73318
::Chemical formula: C10H13N5O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.27 ng/mL
SpecificityNatural and recombinant General 8-Oxo-2'-deoxyguanosine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias Symbols8OHDG, 8-Oxo-2'-deoxyguanosine, 8OHDG, 8-Hydroxydeoxyguanosine
Protein Name8OHDG
Description of Target8-Oxo-2'-deoxyguanosine (8-oxo-dG) is an oxidized derivative of deoxyguanosine. 8-oxo-dG is one of the major products of DNA oxidation. Concentrations of 8-oxo-dG within a cell are a measurement of oxidative stress.
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