Catalog No: AMM99021
Price: $219.00
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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5-Methylcytosine Antibody (AMM99021)

100% of 100
Datasheets/ManualsPrintable datasheet for AMM99021
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Zebrafish, Dog, Pig, Chicken
Product FormatLiquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.
ApplicationFC, IHC-FP, ICC, IP, CHIP, RIA, IF
Additional InformationSterility Test: 0 CFU (on blood agar).
Reconstitution and StorageReady for usage. For longer periods of storage, store at -20C. Can be stored 1 month at 4C.Avoid repeat freeze-thaw cycles.
PurificationProtein A ceramic hyper DF affinity chromatography
Concentration1 mg/ml
Purity> 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)
Intended UseFor research use only.

Conformation-selective methylation of geminivirus DNA. J Virol. 85, 12001-12 (2011) 21835804

Dynamic heterogeneity of DNA methylation and hydroxymethylation in embryonic stem cell populations captured by single-cell 3D high-content analysis. Exp Cell Res. 332, 190-201 (2015). 25700729

Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446-54 (2013). 23739127

Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). 23394161

Global acetylation and methylation changes predict papillary urothelial neoplasia of low malignant potential recurrence: a quantitative analysis. Int J Immunopathol Pharmacol. 24, 489-97 (2011) 21658323

Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500-8 (2008). 18448484

Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533-8 (2012). 22880797

Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799-803 (2012). 22263690

Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722-35 (2011). 21874386

Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780-7 (2008). 17936611

Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays. BMC Mol Biol. 12, 49 (2011). 22098709

Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115-21 (2009). 18752633

Physical localization and DNA methylation of 45S rRNA gene loci in Jatropha curcas L. PLoS One. 8, e84284 (2014). 24386362

PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 27, 46-56 (2018). 29324254

Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports. 9, 329-341 (2017). 28579394

Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers. Oncotarget. 8, 57278-57301 (2017). 28915670

Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372-80 (2011). 21497756

Single cell epigenetic visualization assay. Nucleic Acids Res. (2021). 33511400

Siyuan Chang, et al. Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment. 452 (2020). 32944000

Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77-88 (2013). 23592032

The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice. EMBO J. 34, 748-58 (2015). 25519955

Utility of 5-Methylcytosine Immunohistochemical Staining to Assess Global DNA Methylation and Its Prognostic Impact in MDS Patients. Asian Pac J Cancer Prev. 18, 3307-3313 (2017). 29286224

Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). 18922183

Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215-28 (2010). 20482652

Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25-34 (2008). 18239133

Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938-43 (2008). 18593814

ReferenceMaki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Walsh, T. K., et al., Insect Mol Biol. 19 Suppl 2:215-28 (2010).
Brena, R. M., et al., Methods in Molecular Biology, 507, Part IV, 257-269 (2009).
Zhang, W., et al., Plant Cell. 20(1):25-34 (2008).
Zhang, W., et al, Genome Res. 18(12):1938-1943 (2008).
Jensen, T. J., et al., Carcinogenesis.29(8):1500-1508 (2008).
Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Vrba, L., et al., BMC Genomics. 15;9:486 (2008).
Vinsky, M. D., et al., Reprod Fertil Dev. 19(5):603-611 (2007).
Novak, P., et al., Cancer Res. 66(22):10664-10670 (2006).
Description of TargetMethylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
  1. What is the species homology for "5-Methylcytosine Antibody (AMM99021)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Zebrafish, Dog, Pig, Chicken".

  2. How long will it take to receive "5-Methylcytosine Antibody (AMM99021)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".

  3. What buffer format is "5-Methylcytosine Antibody (AMM99021)" provided in?

    This item is provided in "Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "5-Methylcytosine Antibody (AMM99021)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "5-Methylcytosine Antibody (AMM99021)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "5-Methylcytosine Antibody (AMM99021)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "5-Methylcytosine Antibody (AMM99021)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "5-Methylcytosine Antibody (AMM99021)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with ""?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with ""?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with ""?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with ""?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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