5-Methylcytosine Antibody (AMM99021)

Catalog No: AMM99021

Size:50ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Zebrafish, Dog, Pig, Chicken
• Product Format: Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.
• Clonality: Monoclonal
• Clone: 33D3
• Isotype: IgG1
• Host: Mouse
• Application: FC, IHC-FP, ICC, IP, CHIP, RIA, IF
• Additional Information: Sterility Test: 0 CFU (on blood agar).
  
• Reconstitution and Storage: Ready for usage. For longer periods of storage, store at -20C. Can be stored 1 month at 4C.Avoid repeat freeze-thaw cycles.
• Purification: Protein A ceramic hyper DF affinity chromatography
• Concentration: 1 mg/ml
• Purity: > 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)
• Intended Use: For research use only.
• Publications:

  Conformation-selective methylation of geminivirus DNA. J Virol. 85, 12001-12 (2011) 21835804

  Dynamic heterogeneity of DNA methylation and hydroxymethylation in embryonic stem cell populations captured by single-cell 3D high-content analysis. Exp Cell Res. 332, 190-201 (2015). 25700729

  Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446-54 (2013). 23739127

  Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). 23394161

  Global acetylation and methylation changes predict papillary urothelial neoplasia of low malignant potential recurrence: a quantitative analysis. Int J Immunopathol Pharmacol. 24, 489-97 (2011) 21658323

  Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500-8 (2008). 18448484

  Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533-8 (2012). 22880797

  Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799-803 (2012). 22263690

  Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722-35 (2011). 21874386

  Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780-7 (2008). 17936611

  Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays. BMC Mol Biol. 12, 49 (2011). 22098709

  Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115-21 (2009). 18752633

  Physical localization and DNA methylation of 45S rRNA gene loci in Jatropha curcas L. PLoS One. 8, e84284 (2014). 24386362

  PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 27, 46-56 (2018). 29324254

  Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports. 9, 329-341 (2017). 28579394

  Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers. Oncotarget. 8, 57278-57301 (2017). 28915670

  Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372-80 (2011). 21497756

  Single cell epigenetic visualization assay. Nucleic Acids Res. (2021). 33511400

  Siyuan Chang, et al. Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment. 452 (2020). 32944000

  Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77-88 (2013). 23592032

  The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice. EMBO J. 34, 748-58 (2015). 25519955

  Utility of 5-Methylcytosine Immunohistochemical Staining to Assess Global DNA Methylation and Its Prognostic Impact in MDS Patients. Asian Pac J Cancer Prev. 18, 3307-3313 (2017). 29286224

  Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). 18922183

  Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215-28 (2010). 20482652

  Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25-34 (2008). 18239133

  Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938-43 (2008). 18593814

• Reference: Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
  Walsh, T. K., et al., Insect Mol Biol. 19 Suppl 2:215-28 (2010).
  Brena, R. M., et al., Methods in Molecular Biology, 507, Part IV, 257-269 (2009).
  Zhang, W., et al., Plant Cell. 20(1):25-34 (2008).
  Zhang, W., et al, Genome Res. 18(12):1938-1943 (2008).
  Jensen, T. J., et al., Carcinogenesis.29(8):1500-1508 (2008).
  Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
  Vrba, L., et al., BMC Genomics. 15;9:486 (2008).
  Vinsky, M. D., et al., Reprod Fertil Dev. 19(5):603-611 (2007).
  Novak, P., et al., Cancer Res. 66(22):10664-10670 (2006).

Target Info

• Description of Target: Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.