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5-Hydroxytryptophan ELISA Kit (OKCD02250)

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Gene Symbol:
5-HTP
Alias Symbols:
5-hydroxy-L-tryptophan; L-5-Hydroxytryptophan; Oxitriptan; 4350-09-8; 5-hydroxytryptophan
Description of Target:
5-Hydroxytryptophan is an aromatic amino acid with antidepressant activity. In vivo, oxitriptan (or 5-hydroxytryptophan) is converted into 5-hydroxytryptamine (5-HT or serotonin) as well as other neurotransmitters. Oxitriptan may exert its antidepressant activity via conversion to serotonin or directly by binding to serotonin (5-HT) receptors within the central nervous system (CNS). Endogenous oxitriptan is produced from the essential amino acid L-tryptophan. The exogenous therapeutic form is isolated from the seeds of the African plant Griffonia simplicifolia.
Protein Name:
5-Hydroxytryptophan
Species Reactivity:
Pan-Species
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
1.93 ng/mL
Kit Range:
4.94 - 400 ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level target.
Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level target using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology 5-HTP ELISA Kit (OKCD02250) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-5-HTP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 5-HTP and incubated. The 5-HTP found in the sample or standards competes with the biotinylated 5-HTP for limited binding sites on the immobilized anti-5-HTP antibody. Wells are washed and Avidin-HRP conjugate is added, incubated then washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 5-HTP captured in the well and inversely proportional to the amount of 5-HTP which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-5-Hydroxytryptophan Microplate96 Wells (12 x 8 Well Strips)
5-Hydroxytryptophan Lyophilized Standard2
100X 5-Hydroxytryptophan Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-104%98-105%95-102%86-103%
EDTA plasma(n=5)87-101%86-101%99-105%78-96%
heparin plasma(n=5)80-90%80-96%96-103%91-99%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10598
EDTA plasma(n=5)99-105102
heparin plasma(n=5)90-9794
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Competitive Inhibition Immunoassay
Additional Information:
CAS: 4350-09-8
PubChem: 439280
Protocol:
Tips Information:

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