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5-Hydroxyeicosatetraenoic Acid ELISA Kit (OKCD02249)

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Gene Symbol:
Alias Symbols:
5(S)-HETE; 5-Hydroxyeicosatetraenoic acid; 5S-HETE; 5-Hete; 5-Hydroxyeicosatetraenoate; 5-Hydroxy-6,8,11,14-eicosatetraenoic acid
Protein Name:
5-Hydroxyeicosatetraenoic Acid
Sample Type:
Serum, plasma and other biological fluids
3.95 ng/mL
Kit Range:
9.88 - 800 ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level target.
Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level target using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology 5-HETE ELISA Kit (OKCD02249) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-5-HETE antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 5-HETE and incubated. The 5-HETE found in the sample or standards competes with the biotinylated 5-HETE for limited binding sites on the immobilized anti-5-HETE antibody. Wells are washed and Avidin-HRP conjugate is added, incubated then washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 5-HETE captured in the well and inversely proportional to the amount of 5-HETE which was contained in the sample or standard.
Kit Component:
Anti-5-Hydroxyeicosatetraenoic Acid Microplate96 Wells (12 x 8 Well Strips)
5-Hydroxyeicosatetraenoic Acid Lyophilized Standard2
5-Hydroxyeicosatetraenoic Acid Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Kit Linearity:
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)89-103%96-104%80-92%83-90%
heparin plasma(n=5)78-90%91-102%81-102%85-94%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)84-9792
heparin plasma(n=5)82-9590
Kit Detection Method:
Colorimetric, OD450 nm
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Competitive Inhibition Immunoassay
Additional Information:
CAS: 70608-72-9
PubChem: 5280733

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