Catalog No: OKCD02249
Size:96WELLS
Price: $775.00
SKU
OKCD02249
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5-Hydroxyeicosatetraenoic Acid ELISA Kit (OKCD02249)

Datasheets/ManualsPrintable datasheet for 5-Hydroxyeicosatetraenoic Acid ELISA Kit (OKCD02249)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)86-104%84-104%90-98%98-105%
EDTA plasma(n=5)89-103%96-104%80-92%83-90%
heparin plasma(n=5)78-90%91-102%81-102%85-94%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) and unlabeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample.
ELISA Kit Range9.88-800ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-10399
EDTA plasma(n=5)84-9792
heparin plasma(n=5)82-9590
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxyeicosatetraenoic Acid (5-HETE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxyeicosatetraenoic Acid (5-HETE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-5-Hydroxyeicosatetraenoic Acid Microplate96 Wells (12 x 8 Well Strips)
5-Hydroxyeicosatetraenoic Acid Lyophilized Standard2
5-Hydroxyeicosatetraenoic Acid Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationCAS: 70608-72-9
PubChem: 5280733
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity3.95 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene Symbol5-HETE
Alias Symbols5(S)-HETE; 5-Hydroxyeicosatetraenoic acid; 5S-HETE; 5-Hete; 5-Hydroxyeicosatetraenoate; 5-Hydroxy-6,8,11,14-eicosatetraenoic acid
Protein Name5-Hydroxyeicosatetraenoic Acid
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