4E-BP1 Antibody (OAAD00440)

Catalog No: OAAD00440

Size:0.1 mg

western blot analysis using anti-Human 4E-BP1 monoclonal antibody against recombinant 4E-BP1 (
Lane 1) and A431 cell lysate (
Lane 2).

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 9E12D9
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Formulation: Antibodies are purified by protein A affinity chroma- tography
• Application Info: Western Blot: 1: 500- 1: 1, 000.
  ELISA: Propose dilution 1: 10, 000.
  Determining optimal working dilutions by titration test.
• Reference: 1. Pause, A. et al. 1994.Nature. 371:762-767.
  2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240- 10247.

Target Info

• Gene Symbol: Eif4ebp1
• Alias Symbols: PH, 4e-bp, 4e-bp1, PHAS-I, AA959816
• NCBI Gene Id: 13685
• Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Description of Target: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1), also called ELF4EBP1/BP- 1/PHAS-I , which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is depen- dent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
• Uniprot ID: Q60876