Catalog No: OCPB00172
Size:100UG
Price: $156.00
SKU
OCPB00172
Availability: Discontinued
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293 Lysate (OCPB00172)
HEK293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HEK293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Datasheets/Manuals | Printable datasheet for 293 Lysate (OCPB00172) (OCPB00172) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. HEK293 lysate is supplied in SDS sample buffer containing 50 mM DTT. |
Host | Human |
Application | Western blot |
Reconstitution and Storage | 2°C to 8°C|-80°C |
Concentration | 2 mg/ml |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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