Catalog No: OKBA00028
Size:96 Wells
Price: $225.00
SKU
OKBA00028
Availability: Discontinued
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25-OH Vitamin D Total ELISA Kit (Human) : 96 Wells (OKBA00028)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 hours
ELISA Kit Range0-150 ng/mL
ELISA Kit Component1. Vit D Calibrators - 1ml/vial.
2. Vit D Controls - 1ml/vial Two (2) vials containing human serum reference controls at concentration established (exact value listed on label). A preservative has been added. Store at 2-8 C.
3. Vit D Releasing Agent - 12 ml/vial One (1) vial containing Vitamin D binding protein releasing agents. Store at 2-8 C.
4. Vit D Enzyme Reagent - 12 ml/vial One (1) vial containing 25-OH Vitamin D3 (Analog)-horseradish peroxides (HRP) conjugate in a protein-stabilizing matrix. Store at 2-8 C.
5. Vit D Antibody Coated Plate - 96 wells One 96-well microplate coated with < 1.0 ug/ml anti-Vitamin D sheep IgG and packaged in an aluminum bag with a drying agent. Store at 2-8 C.
6. Wash Solution Concentrate - 20 ml/vial One (1) vial containing a surfactant in buffered saline. A preservative has been added. Store at 2-8 C.
7. Substrate Reagent - 12 ml/vial One (1) vial containing tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in buffer. Store at 2-8 C.
8. Stop Solution - 8 ml/vial One (1) vial containing a strong acid (H2SO4). Store at 2-8 C.
9. Product Insert.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum
Sensitivity1.14 ng/mL
Alias Symbols25OHVD, 25-OH Vitamin D, 25OHVD, Calcifediol, Calcidiol
Description of TargetLow blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases
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