Catalog No: OKBA00028
Size:96 Wells
Price: $225.00
SKU
OKBA00028
Availability: Discontinued
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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COA Datasheet | Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Predicted Species Reactivity | Human |
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Application | ELISA |
ELISA Kit Detection Method | Colorimetric, OD 450 nm |
ELISA Kit Duration | ~ 3 hours |
ELISA Kit Range | 0-150 ng/mL |
ELISA Kit Component | 1. Vit D Calibrators - 1ml/vial. 2. Vit D Controls - 1ml/vial Two (2) vials containing human serum reference controls at concentration established (exact value listed on label). A preservative has been added. Store at 2-8 C. 3. Vit D Releasing Agent - 12 ml/vial One (1) vial containing Vitamin D binding protein releasing agents. Store at 2-8 C. 4. Vit D Enzyme Reagent - 12 ml/vial One (1) vial containing 25-OH Vitamin D3 (Analog)-horseradish peroxides (HRP) conjugate in a protein-stabilizing matrix. Store at 2-8 C. 5. Vit D Antibody Coated Plate - 96 wells One 96-well microplate coated with < 1.0 ug/ml anti-Vitamin D sheep IgG and packaged in an aluminum bag with a drying agent. Store at 2-8 C. 6. Wash Solution Concentrate - 20 ml/vial One (1) vial containing a surfactant in buffered saline. A preservative has been added. Store at 2-8 C. 7. Substrate Reagent - 12 ml/vial One (1) vial containing tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in buffer. Store at 2-8 C. 8. Stop Solution - 8 ml/vial One (1) vial containing a strong acid (H2SO4). Store at 2-8 C. 9. Product Insert. |
Reconstitution and Storage | Store as indicated in product manual. |
Sample Type | Serum |
Sensitivity | 1.14 ng/mL |
Alias Symbols | 25OHVD, 25-OH Vitamin D, 25OHVD, Calcifediol, Calcidiol |
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Description of Target | Low blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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