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Catalog No: OKCD02248
Size:96WELLS
Price: $850.00
SKU
OKCD02248
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2-Arachidonoylglycerol ELISA Kit (OKCD02248)

Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-98%97-104%88-96%84-101%
EDTA plasma(n=5)87-99%84-104%92-101%85-95%
heparin plasma(n=5)89-103%93-104%90-98%79-88%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 2-AG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 2-AG analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 2-AG in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 2-AG in the sample.
ELISA Kit Range3.70-300ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)79-9487
EDTA plasma(n=5)94-10197
heparin plasma(n=5)87-10391
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2-Arachidonoylglycerol (2-AG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2-Arachidonoylglycerol (2-AG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-2-Arachidonoylglycerol Microplate96 Wells (12 x 8 Well Strips)
2-Arachidonoylglycerol Lyophilized Standard2
100X 2-Arachidonoylglycerol Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 53847-30-6
PubChem: 5282280
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.41 ng/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene Symbol2-AG
Alias Symbols2-arachidonoylglycerol; 2-Arachidonylglycerol; 2-AG; 2-Arachidonyl-glycerol; 2-Arachidonoyl-glycerol; 2-Arachidonoyl Glycerol
Protein Name2-Arachidonoylglycerol
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