10X PBS pH 7.2 with Azide (OORA00260)

Catalog No: OORA00260

Size:1L

Product Info

• Product Format: Liquid (sterile filtered)
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
• Reconstitution and Storage: Store container at room temperature (18 to 26°C). If desired, the solution may be stored at 4°C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18 to 26°C) to restore solubility of some salts. Expiration date is one (1) year from date of opening.
• Concentration: 10X
• Specificity: Tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
• Application Info: 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
• Purification: Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers.

Target Info

• Description of Target: Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution). PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration.