10X PBS pH 7.2 (OORA00259)

Catalog No: OORA00259

Size:1L

Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

Product Info

• Product Format: Liquid
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay, Flow cytometry, Immunofluorescence, Western blot
• Reconstitution and Storage: Store container at room temperature (18 to 26°C). If desired, the solution may be stored at 4°C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18 to 26°C) to restore solubility of some salts. Expiration date is six (6) months from date of opening container.
• Application Info: This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished pharmaceutical grade water (WFI).
• Purification: 10X PBS buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.