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10X Antigen Retrieval Solution pH 9.0 for Immunohistochemistry (OOMB00007)

Catalog#: OOMB00007
Domestic: within 1 week delivery | International: 1 week
Antigen Retrieval Solution is a Tris/EDTA, pH 9.0, intended for Heat Induced Epitope Retrieval (HIER) in tissue sections prior to adding primary antibodies. This solution is designed for unmasking a wide variety of antigens located on cell membrane, in the cytoplasm and in cell nuclei.
This solution allows for significant increase of specific staining signal without producing non-specific background staining in specimens over-fixed with formalin as well as in long-stored archived tissues.
To make a working solution dilute Antigen Retrieval Solution 10X concentrate to 1X concentration with distilled water.
First deparafinize formalin-fixed paraffin-embedded tissue sections and then immerse them into Antigen Retrieval Solution heated between 95 and 100C for 10 minute, remove from heat and let Antigen retrieval Solution with immersed slides to cool down for 20-40 minutes. Pressure cooker or vegetable steamer can be used for retrieval procedure.
More Information
Product Format Liquid.(filtered) - Thimerosal is added as an antimicrobial agent
Application IHC
Reconstitution and Storage Store solution at room temperature. Expiration date is twelve (12) months from date of receipt if properly handled and stored.
Datasheets/Manuals Printable datasheet for OOMB00007
Purification This product was aseptically filtered through a Millipore 0.22-micron filter into clean containers.
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