Catalog No: OORA00271
Size:100ML
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Product Format | Liquid (sterile filtered) |
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Conjugation | Unconjugated |
:: | Label: Unconjugated Preservative: None Sterilization: This product was aseptically filtered through a Millipore 0.22-micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria. |
Reconstitution and Storage | Store container at room temperature (18-26°C) prior to opening. If desired, the solution may be stored at 4°C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18-26°C) to restore solubility of some salts. 1.0 M HEPES pH 7.2 is a concentrated stock solution and should be diluted appropriately with molecular biology grade water or equivalent to its final working concentration. |
Concentration | 1.0 M |
Purification | 1.0 HEPES pH 7.2 was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 1.0 M HEPES pH 7.2 was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria. |
Preparation | Prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water. |
Description of Target | HEPES is a useful buffer for pH's ranging from 6.8-8.2. This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration. 1.0 M HEPES pH 7.2 is ideal for investigators in Molecular Biology, Cell Biology, and Immunology researchers. |
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- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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