ZDHHC8 Antibody (OAEB01426)

Catalog No: OAEB01426 (Formerly GWB-C6566B)

Size:100UG

OAEB01426 (0.1ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ZDHHC8
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-QRDHPQLKTPPSK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3ug/ml.
  An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Reference: * Mukai J, Liu H, Burt RA, Swor DE, Lai WS, Karayiorgou M, Gogos JA.; Evidence that the gene encoding ZDHHC8 contributes to the risk of schizophrenia.; Nat Genet. 2004 Jul;36(7):725-31. Epub 2004 Jun 6.; Pubmed 15184899

Target Info

• Gene Symbol: Zdhhc8|ZDHHC8
• Gene Full Name: zinc finger DHHC-type palmitoyltransferase 8|zinc finger, DHHC domain containing 8
• Alias Symbols: D16H22S1738E;DHHC8;DHHC-8;DHHC-containing protein 8;E330009O14Rik;membrane-associated DHHC8 zinc finger protein;Op53c;Op53c05;palmitoyltransferase ZDHHC8;probable palmitoyltransferase ZDHHC8;ZDHHCL1;zinc finger DHHC domain-containing protein 8;zinc finger DHHC-type containing 8;zinc finger protein 378;zinc finger, DHHC domain containing 8;zinc finger, DHHC domain like containing 1;zinc finger, DHHC-type containing 8;zinc finger, DHHC-type containing 8-like;ZNF378.
• NCBI Gene Id: 27801|29801|303796
• Protein Name: Probable palmitoyltransferase ZDHHC8
• Uniprot ID: Q9ULC8
• Protein Accession #: NP_037505.1
• Molecular Weight: 81.4