Catalog No: OKEH02349
Size:96T
Price: $625.00
SKU
OKEH02349
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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YWHZ ELISA Kit (Rat) : 96 Wells (OKEH02349)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology YWHZ ELISA Kit (Rat) (OKEH02349) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YWHZ has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YWHZ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample YWHZ captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
YWHZ Microplate96 Wells (12 x 8 Well strips)
YWHZ Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated AKR1C1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.68 pg/mL
SpecificityNatural and recombinant Rat 14-3-3 protein zeta/delta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolYwhaz
Gene Full Nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
Alias Symbols14-3-3 protein zeta/delta;14-3-3z;KCIP-1;mitochondrial import stimulation factor S1 subunit;protein kinase C inhibitor protein 1;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.
NCBI Gene Id25578
Protein NameP63102
Description of Target14-3-3 protein zeta/delta
Uniprot IDhttps://www.uniprot.org/uniprot/P63102
Protein Accession #NP_037143.2
Nucleotide Accession #NM_013011.3
Protein Size (# AA)245
Molecular Weight28 kDa
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